Procedures for Collection of Induced Sputum Specimens From Children

Procedures for collection of induced sputum specimens from children.

In most settings, sputum is not routinely collected for microbiological diagnosis from children with lower respiratory disease. To evaluate whether it is feasible and diagnostically useful to collect sputum in the Pneumonia Etiology Research for Child Health (PERCH) study, we reviewed the literature on induced sputum procedures. Protocols for induced sputum in children were collated from publis...

Induced Sputum in Children

Background: Sputum induction is a noninvasive method for the assessment of airway infl ammation. Objectives: To evaluate the safety of the procedure and the clinical predictors of successful induction, and to analyze the relationship between sputum cell counts and clinical features in asthmatic and nonasthmatic children. Methods: We reviewed sputum inductions performed in our department between...

Specimens from the Hunterian Collection.

Magnetic bead protocol for culturing Mycobacterium tuberculosis from sputum specimens.

A magnetic bead protocol and a standard centrifugation protocol yielded Mycobacterium tuberculosis in 40/50 sputum specimens in 12 ± 1 days and 11 ± 2 days, respectively (P > 0.05 by Student's t test). Manipulation took 35 ± 5 min and 45 ± 10 min, respectively (P < 0.05). The magnetic bead protocol could advantageously replace centrifugation for culturing M. tuberculosis from sputum.

Safety of Induced Sputum Collection in Children Hospitalized With Severe or Very Severe Pneumonia

Background. Induced sputum (IS) may provide diagnostic information about the etiology of pneumonia. The safety of this procedure across a heterogeneous population with severe pneumonia in low- and middle-income countries has not been described. Methods. IS specimens were obtained as part a 7-country study of the etiology of severe and very severe pneumonia in hospitalized children <5 years of...